The first part of the fluorometer is very similar to the spectrophotometer, and consists of a light source and a filter or monochromator to select out a defined group of excitation wavelengths, which are then directed into a sample. Used thermo scientific fluoroskan ascent microplate. Fluorescence intensities were measured on fluoroskan ascent microplate fluorometer. The fluoroskan ascent fl is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric applications even in the same run. Our exclusive contracts with our clients yield a wide range of used microplate readers from a number of respected oems, including perkin elmer, tecan, molecular devices, biotek instruments, packard automation, labsystems and more.
Perform a variety of research applications with the compact, robust thermo scientific. In many molecular biology applications the detection and quantitation of small amounts of dsdna is very important. Ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as. Microplate fluorometers for a variety of uses in the life science field, including dna, rna, and. A filter fluorometer will use filters while a spectrofluorometer will use grating monochromators. Comes with computer and software, plus some manuals. Cisplatin resistance is multifactorial but can be associated with increased dna repair capacity, mutations in p53 or loss of dna mismatch repair. The fluoroskan ascent is a highquality microplate fluorometer intended for laboratory. Thermo scientific accessories for fluoroskan ascent fl microplate fluorometer and luminometer perform a range of fluorometric and luminometric research applications with thermo scientific fluoroskan ascent fl, offering fast read speed and topbottom plate reading. Regulation of endothelial permeability and transendothelial. The fluoroskan ascent fl fluoroskan ascent can carry out both top and bottom reading. Featuring excellent optical performance, the fluoroskan microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studies, molecular interaction studies, nucleic acid quantification, as well as reporter gene, fluorometric kinase, immunological, and cellbased assays. Thermo labsystems multiskan ascent 354 microplate plate reader. Over 10 million scientific documents at your fingertips.
Decreased transcriptioncoupled nucleotide excision repair. We already reported that endothelial cell integrity is tightly regulated by the balanced coactivation of p38 and erk pathways. Spectral peaks may shift as a function of temperature, ph, solvent, or coupling chemistry. Analytical tlc was performed on readytouse plates with silica gel 60 merck. Prices are indicative only and may vary by country, with changes to the cost of raw materials and. Super high resolution for single moleculesequencebased. Fluoroskan ascent fl fluorometer with three dispensers, 5210463, ca95043050, each.
Fluoroskan ascent fl is a microplate fluorometer and luminometer with excellent optical performance for a variety of research applications, offering versatile plate formats, fast reading speed, up to three dispensers and top bottom reading of plates. The thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. Fluorescence measurements were carried out in a fluoroskan ascent microplate fluorometer using excitation and emission wavelengths of 355 and 405 nm, respectively. The irrelevant mirnas celmir243 and athmir416 were used as negative controls. F luoroskan, fluoroskan fl, and l uminoskan microplate readers. Impact of dabigatran on a large panel of routine or specific. Microplate instrumentation mib application laboratory, thermo fisher scientific, vantaa, finland introduction the picogreen reagent is an ultra. Fluoroskan fl microplate fluorometer and luminometer, 100240 v, 5060 hz with two dispensers 5200222 includes pc software and filter pairs. The position of the whole optic unit is easily changed. The fluoroskan ascent from labsystems is a microplate fluorometer which. Super high resolution for single moleculesequencebased typing of classical hla loci at the 8digit level using next generation sequencers. Thermo scientific fluoroskan ascent or fluoroskan ascent fl, this reagent provides rapid and easy determination of dsdna, offering ultimate sensitivity and a wide dynamic range. One of the most commonly used classes of anticancer drugs presently in clinical practice is the platinumbased drugs, including cisplatin.
Instrument fluoroskan microplate fluorometer 5200110 fluoroskan fl microplate fluorometer and luminometer 5200220 reagents quantit dsdna assay kit, high sensitivity q33120 microplates. The direct illumination optics of the fluoroskan ascent produces a highly focused light beam. Statins reduce lipopolysaccharideinduced cytokine and. The fluoroskan ascent fl is a combined highquality microplate fluorometer and luminometer intended for laboratory research use by professional personnel. Luminometric measurement principle fluoroskan ascent fl. Fluorometric gfp measurement and luminometric luciferase measurement from the same well occurs quickly due to the versatility of the assay programming.
Oct 12, 2017 luciferase measurements were carried out as described using a fluoroskan ascent fl microplate fluorometer and luminometer. A fluorometer sometimes fluorimeter is a spectrometer designed to measure this phenomenon. May 06, 2016 one hundred microliters of sample was collected from the abluminal compartment at the end of 30 minutes and measured fluorometrically at 485520 nm excitationemission using fluoroskan ascent fl microplate fluorometer and luminometer vantaa, finland. Molecule contains electrons, electrons and non bonding n electron. Download pdf copy thermo scientific fluoroskan ascent microplate fluorometer is a compact and robust instrument with excellent optical performance for a variety of research applications. Best suited for research environments, applications include fluorometric protein, enzyme, and kinase studies. L with 50 mm tris buffer ph 7 ionic strength adjusted to 100 mm with nacl with a fluorescein filter set ex. Measurement with the fluoroskan ascent fl and fluoroskan ascent occurs in a narrow angle. Labsystems multiskan ex manual 2806100, view, unable labsystems to connect to commerce server, parts for multiskan rc ms ex from thermo scientific brand microplate readers manual. An optical system based in fiberless direct illumination gives the instrument a wide range of applications. Fluoroskan fl microplate reader fluorometer and luminometer fluoroskan microplate reader dedicated fluorometer luminoskan microplate reader dedicated luminomter on board dispensing is important for flash chemistry such as atp measurement or calcium flux assays the instrument includes incubation and can be ordered with up to two dispensers. Thermo scientific accessories for fluoroskan ascent fl. Labsystems now thermo 374 fluoroskan ascent fluorescence microplate reader itemspecific notes. The thermo scientific fluoroskan ascent fl is a compact microplate fluorometer and luminometer.
The labsystems fluoroskan ascent fl is microplate fluorometer and. A calibrated automated tool to assess the thrombin. In his honor there is a board of recognition by the state of aguascalientes at the base of the column of the exedranext to the fountain from a spring dedicated to this musical poet, in the city of aguascalientes where he grew up and first studied music. The emission filter slots 7 and 8 are reserved only. Fluoroskan ascent microplate fluorometer fluoroskan ascent fl combined microplate fluorometer and luminometer reads 84well plates topbottom reading robotic compatible up to three dispensers incubation and shaking high sensitivity luminoskan ascent microplate luminometer reads 84well plates high. This microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studie. A fluorescencebased protocol for quantifying angiotensin. Fluorometers fluorescence spectrophotometers a fluorescence spectrophotometer or fluorometer, fluorospectrometer, or fluorescence spectrometer measures the fluorescent light emitted from a sample at different wavelengths, after illumination with a xenon flash lamp. Fluoroskan fl microplate fluorometer and luminometer.
Cell phenotype was investigated by both microplate fluorometer and epifluorescence. Crude and purified proteasome activity assays are affected by type. Pdf fluorescence microplate assay for the detection of oxidative. Equipnet is the worlds leading provider of used microplate readers and a number of other used equipment. Upload files that contain software or other material protected by intellectual property laws or by rights. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic measurements from the very start of the reaction. Fluoroskan ascent microplate fluorometer according to the manufacturers protocol.
Samples were tested in triplicate and calibrated as previously described. Fluoroskan ascent microplate fluorometer pdf thermo scientific fluoroskan ascent microplate fluorometer. If you dont see the used microplate reader that youre. Improved method to retain cytosolic reporter protein. Compare fluoroskan ascent series microplate readers from thermo fisher scientific on.
As nouns the difference between fluorimeter and fluorometer is that fluorimeter is a fluorometer while fluorometer is an instrument used to detect and measure fluorescence. Up to three reagent dispensers can be fitted onboard, making the reagent addition simple and highly accurate. Thermo scientific fluoroskan ascent microplate fluorometer. The thermo scientific fluoroskan ascent fl microplate reader is. Loss of endothelial cell integrity and selective permeability barrier is an early event in the sequence of oxidantmediated injury and may result in atherosclerosis, hypertension and facilitation of transendothelial migration of cancer cells during metastasis. Automated selfdiagnostics and automatic power save functions offer timesaving convenience. View pdf catalogues and other online documentation prices are pretax.
Ogr1 probe was synthesized by using an applied biosystems 394 dnarna synthesizer. For applications such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and. Standard factoryinstalled filter pair for fluoroskan and fluoroskan fl instruments. The g126v mutation in the mouse prion protein hinders. Molecular devices spectramax gemini xs for sale labx. The instrument is controlled with the flexible thermo scientific ascent software. Ascent fl fluoroskan ascent can carry out both top and bottom reading. Labsystems fluoroskan ascent fl equipment information msu. Fluoroskan ascent microplate fluorometer thermo fisher scientific with black, nonbinding 384 well plates greiner at a reaction volume of 60. The difference between them is the way they select the wavelengths of incident light. May 25, 2019 here my blog where i share pdf files with my readers. Thermo fisher scientific the 374 fluoroskan ascent microplate fluorometer features excellent optical performance. Used labsystems fluoroskan ascent 2024129 fluorometer. Includes mtx spectrosoft software installation cd, dongle, cover, and cordscables as pictured.
There are two basic types of fluorometers, the filter fluorometer and the spectrofluorometer. The fluoroskan ascent figure 4 is an advanced microplate fluorometer with high sensitivity for a wide variety of fluorometric applications. Thermofisher scientific fluoroskan ascent fl fluorometer. Cell culture all melanoma cell lines in this study were established from metastasis of patients with stage iii or iv melanoma according to ajcc as previously described 17. It is measurement of fluorescence intensity at a particular wavelength wit the help of a filter fluorimeter or a spectrofluorimeter. Upload files that contain software or other material protected by intellectual property laws or by rights of privacy of publicity unless you own or control the rights thereto or have received all necessary consents. Each well was scanned by measuring the intensity of each of 25 squares of 1mm2 area arranged in a 5. Therefore, the assumption that all statin compounds will have the same effect within the central. High sensitivity luminometric and fluorometric assays in a.
Antioxidants improve the viability of stored adult retinal pigment. Thermo scientific luminoskan ascent thermo scientific. The thermo scientific fluoroskan fl is a compact and robust microplate fluorometer and luminometer, offering simplicity and reliability for increased efficiency of workflows. Microplate fluorometers for a variety of uses in the life science field, including dna, rna, and enzyme studies, feature enhanced reading speeds. Tcdd was added to lmat cells that had been loaded with 5.
In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Shop online for a wide selection of thermo scientific fluoroskan ascent microplate fluorometer perform a variety of research applications with the. Fluoroskan, fluoroskan fl and luminoskan key features. The only components required are a standard of known concentration and a blank. Fluoroskan ascent fluorescence cf cell reading fluorescence. The fluoroskan fl microplate fluorometer and luminometer is capable of reading a 96well plate in just 18 seconds, essential for kinetic applications. Fluoroskan ascent microplate fluorometer thermo fisher scienti. Thermo labsystems ascent family covers both fluorometry and luminometry with. The 374 fluoroskan ascent microplate fluorometer features excellent optical performance. Thermo scientific fluoroskan ascent the thermo scientific fluoroskan ascent is a microplate fluorometer for life science research applications, such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and cell based assays. Used thermo fluoroskan ascent 374 fluorometer for sale. The electrons may be present in bonding molecular orbital.
Thermo scientific fluoroskan ascent forums, the thermo scientific fluoroskan ascent is a computercontrolled microplate fluorometer for life science research applications, such as fluorometric protein and other files to download. Fluoroskan ascent microplate fluorometer from thermo. Featuring excellent optical performance, the fluoroskan fl microplate fluorometer and luminometer is ideal for life science fluorometric and luminometric assays. The intensity of fluo3 fluorescence in tcddtreated cells increased over time, and the increase was biphasic. He was married to clementina maurel, next to whom he died in mexico city. Used thermo scientific fluoroskan ascent microplate fluorometer.
Electrophoresis, blotting and immunodetection plate based. This table contains only a partial list of the fluorescent dyes that can be used with the fluoroskan and fluoroskan fl microplate fluorometers. The compact fluoroskan ascent cf is simplified and standardized to focus specifically on cellbased requirements. Thermo scientific fluoroskan ascent, luminoskan ascent and fluoroskan ascent fl fluoroskan ascent luminoskan ascent fluoroskan ascent fl fluorometry excitation wavelength range 320 700 nm 320 700 nm emission wavelength range 360 800 nm 360 670 nm excitation filters up to eight filters in the excitation filter wheel. After a flash of onoff welcome screen, similar to the one shown in figure 1, the screen automatically. Thermo scientific fluoroskan ascent fl the thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. Fluoroskan ascent series microplate readers from thermo. Stimulation of gsh synthesis to prevent oxidative stress. Highthroughput identification of peptide agonists against. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Technical note fluoroskan microplate fluorometer sensitive fluorometric quantification of dna in microplate format materials cat. Measuring fluorescein using the quantus fluorometer.
The fluoroskan ascent flfluoroskan ascent can carry out both top and bottom reading. Cam fluorescence quantification was performed with a microplate fluorometer fluoroskan ascent, thermo scientific. Fluoroskan ascent and fluoroskan ascent fl microplate. It is currently unknown whether statins neurological effects are lipiddependent or due to pleiotropic mechanisms. Fluoroskan ascent microplate fluorometer luminoskan ascent microplate luminometer fluoroskan ascent fl combined microplate. Oct 30, 2015 samples were analyzed for 50 minutes and fluorescence was recorded every 6 seconds with a fluoroskan ascent microplate fluorometer with. The source of the excitation light and the detector of the emission light are both located on the same side of the microplate. Thus, the reader is ideal for applications such as reporter gene and cell adhesion assays, as well as cell proliferation and cytotoxicity assays.
It features compatibility with 1 to 384 well plate formats and pcr plates, up to three onboard dispensers, an incubator and a shaker. Supporting information potent and selective inhibitors of. Hypoxia induces a prothrombotic state independently of the. Portable models are lightweight and compact for use in field studies. The fluoroskan ascent, a dedicated microplate fluorometer, offers high sensitivity for a wide variety of fluorometric applications. Because fluorescein is autofluorescent, no dye chemistries are necessary for quantitation on the quantus fluorometer. Impact of dabigatran on a large panel of routine or. The efficacy of cisplatin therapy is often limited by the emergence of resistant tumours following treatment. Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins. Institute for molecular medicine, university medical center of the johannes gutenberg, university of mainz, 551 mainz, germany. Before the start of the bril formation reaction, the protein in 10 mm sodium acetate bu. Thermo labsystems 374 fluoroskan ascent microplate fluorometer. The fluoroskan flexible design allows for 196 and 384well plate analysis.
Fluoroskan ascent and fluoroskan ascent fl microplate fluorometers, thermo scientific. The thermo scientific fluoroskan ascent fl microplate reader is a. Fluoroskan ascent fl microplate fluorometer and luminometer for both luminometric and fluorometric research applications. Samples were analyzed for 50 minutes and fluorescence was recorded every 6 seconds with a fluoroskan ascent microplate fluorometer with. Dabigatran was tested at nine concentrations ranging from 4. Thermo scientific fluoroskan ascent fl microplate fluorometer and luminometer is ideal for life science research applications. Excellence for fluorometric and luminometric measurements. The fluoroselect fluorometer can be powered with 4 aa alkaline batteries or a power adapter 5vdc. The antiinflammatory effects of statins hmgcoa reductase inhibitors within the cardiovascular system are wellestablished. Fluorescein can be measured using the quantus fluorometer cat. Panax ginseng induces the expression of catsper genes and. Recommended filter pairs for various fluorescent dyes. The thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase. Thermo scientific fluoroskan ascent fl from thermo fisher.
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